Anti-GFP抗體(tǐ)- ChIP Grade (ab290)

産品名稱

Anti-GFP抗體(tǐ)- ChIP Grade

參閱全部 GFP 一(yī)抗

描述

兔多(duō)克隆抗體(tǐ)to GFP - ChIP Grade

宿主

Rabbit

特異性

Anti-GFP antibody - ChIP Grade (ab290) is a highly versatile antibody that gives a strong↑er signal than other anti-GFP antibodies available. On Western blot the an☆tibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion pro‍teins and has also been shown to be useful on mouse sections fi€xed with formalin. In Immunocytochemistry, the antibody gives a very good± signal on recombinant YES-GFP chimeras expressed in COS cells (M÷cCabe et al. 1999 and figure below). It is routinely used in Immunopre×cipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemist ry at 1:200 on whole-mount mouse embryos.

This anti-GFP antibody recognizes the enhanced form of$ GFP as well.

Recognises EYFP (Yellow Fluorescent Protein (YFP), a genetic mutant of green flu©orescent protein (GFP).

ab290 also detects venus YFP.

經測試應用(yòng)

适用(yòng)于: ELISA, ICC/IF, ChIP, IHC-FrFl, Electron Microscopy, IHC-FoFr, ICC, IHC-P​, IHC-Fr, IP, WBmore details

種屬反應性

與反應: Species independent

免疫原

Recombinant full length protein corresponding to GFP✔.

Database link: P42212

陽性對(duì)照(zhào)

Detects 5ng of recombinant GFP (using ECL or ECL Plus) in αunder one minute of exposure to film. Pure GFP protein, or cells k♦nown to overexpress GFP.

常規說(shuō)明(míng)

The total IgG concentration has been determined to be 5 mg/mL. The specific IgπG concentration is unknown. This product should be kept refrigerated at al≥l times whilst in short term storage. Using sterilised equipmen✘t will reduce the risk of bacterial contamination. 

Anti-GFP antibody (ab6556) is the purified version✘ of this antibody (see Related Products).